METHOD FOR VERIFYING A TEMPERATURE MEASUREMENT IN A MICRO-ENVIRONMENT AND SYSTEM FOR VERIFYING A TEMPERATURE MEASUREMENT IN A MICRO-ENVIRONMENT

The present invention pertains to a method suitable for analysing the temperature control of a device which is supposed to establish a defined temperature in a micro-environment, said method comprising a first Optical Temperature Verification step which comprises a) providing one or more thermochromatic liquid crystals in a micro-environment, wherein each thermochromatic liquid crystal has a specific event temperature, b) providing one or more temperature dependent luminophores in a micro-environment, c) varying the temperature in the micro-environments and irradiating the micro-environments with light, d) recording the luminescence of the one or more temperature dependent luminophores when the event temperature of the one or more thermochromatic liquid crystals is reached in the micro-environment and wherein said method preferably comprises a second Optical Temperature Verification step, which comprises the following a) providing one or more temperature dependent luminophores that were used in the First Optical Temperature Verification in a micro-environment, b) varying the temperature of the micro-environments and irradiating the micro-environments with light, c) monitoring the luminescence emitted. The respective method is very suitable to analyse the temperature control of a thermal cycler, in particular during a calibration.