FLUOROGENIC SUBSTRATES, METHODS OF MAKING THE SUBSTRATES, AND METHODS OF DETECTING GLYCOSIDASE ACTIVITIES

FLUOROGENIC SUBSTRATES, METHODS OF MAKING THE SUBSTRATES, AND METHODS OF DETECTING GLYCOSIDASE ACTIVITIES

Fluorogenic substrates, methods of making the fluorogenic substrates, and methods of detecting glycosidase activities (EC3.2.1) are provided. The fluorogenic substrates are composed of a sugar covalently bonded to the 7-hydroxy position of a substituted coumarin sulfonated fluorophore is provided. T...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: NAJAH MAJDI, MAYOT ESTELLE, LADAME SYLVAIN, DREVELLE ANTOINE
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
DERIVATIVES THEREOF
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
NUCLEIC ACIDS
NUCLEOSIDES
NUCLEOTIDES
ORGANIC CHEMISTRY
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
SUGARS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Fluorogenic substrates, methods of making the fluorogenic substrates, and methods of detecting glycosidase activities (EC3.2.1) are provided. The fluorogenic substrates are composed of a sugar covalently bonded to the 7-hydroxy position of a substituted coumarin sulfonated fluorophore is provided. The fluorogenic substrates include sodium -D-cellobioside 6,8-difluoro-7-hydroxycoumarin-4-methanesulfonate; sodium -D-glucoside 6,8-difluoro-7-hydroxycoumarin-4-methanesulfonate; sodium -D-xyloside 6,8-difluoro-7-hydroxycoumarin-4-methanesulfonate; sodium -D-xylobioside 6,8-difluoro-7-hydroxycoumarin-4-methanesulfonate; and sodium -D-xylopolyoside 6,8-difluoro-7-hydroxycoumarin-4-methanesulfonate. The fluorogenic substrates may be made by covalently bonding the sugar moiety to the 7-hydroxy position of the substituted coumarin sulfonated fluorophore by reaction in DMF. Glycosidase activities can be detected in enzymatic extracts, microorganisms, and cells by contacting the extracts, microorganisms, or cells with one of the fluorogenic substrates.