CASSETTE INCLUDING PROMOTER SEQUENCE OF TARGET GENE AND METHOD OF GENE MANIPULATION USING THE SAME

CASSETTE INCLUDING PROMOTER SEQUENCE OF TARGET GENE AND METHOD OF GENE MANIPULATION USING THE SAME

Provided is a cassette for deleting a target gene comprising (a) a promoter-specific homologous region having a sequence identity to a portion of a promoter region of the target gene, wherein the degree of sequence identity is sufficient to drive homologous recombination therebetween, (b) a marker g...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: KIM SUNG-SOO, LIM HUI-SUB, PARK JAEAN, LEE JU-YOUNG, KIM JAE-YOUNG, CHOO JIN-HO, KANG JIN-KYU, KANG CHANG-DUK, KANG HYUN-AH
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:Provided is a cassette for deleting a target gene comprising (a) a promoter-specific homologous region having a sequence identity to a portion of a promoter region of the target gene, wherein the degree of sequence identity is sufficient to drive homologous recombination therebetween, (b) a marker gene operably linked to the promoter-specific homologous region, and (c) a gene-specific homologous region adjacent to 3′-end of the marker gene and having a sequence identity to at least a portion of the target gene, wherein the degree of sequence identity is sufficient to drive homologous recombination therebetween.