NANNOCHLOROPSIS SPLICED LEADER SEQUENCES AND USES THEREFOR

The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts from Nannochloropsis species. In particular, the invention provides nucleic acid composit...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: BROWN ROBERT C, SESHADRI REKHA, SORIAGA LEAH, SCHWARTZ ARIEL S
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The present invention relates to the culture and manipulation of microorganisms for biotech applications, and is based on the discovery and characterization of spliced leader sequences identified in transcripts from Nannochloropsis species. In particular, the invention provides nucleic acid compositions comprising a SL sequence operably linked to a protein-encoding gene. Further provided are compositions and methods for enhanced gene expression in recombinant microorganisms as well as methods for identification and/or isolation of nucleic acid molecules tagged with a spliced leader sequence.