WHOLE GENOME EXPRESSION ANALYSIS SYSTEM

A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer se...

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Bibliographische Detailangaben
Hauptverfasser: JONES JONES C, KAO H. PIN, SANDELL DONALD, OLDHAM MARK, HARDING IAN A, BAHATT DAR, BODEAU JOHN P, ERMAKOV SERGEY V, LIM GARY, BODNER KEVIN, FAWCETT ADRIAN, PATEL JAYNISH, WOUDENBERG TIMOTHY
Format: Patent
Sprache:eng
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Zusammenfassung:A method for simultaneously determining a genetic expression profile for an individual member of a species relative to an entire standard genome for the species. The method can comprise distributing a liquid sample into an array of reaction chambers of a substrate. The array can comprise a primer set and a probe for each polynucleotide target along the entire standard genome. The liquid sample can comprise substantially all genetic material of the member. Each of the reaction chambers can comprise the primer set and the probe for at least one of the polynucleotide targets and a polymerase. The method can further comprise amplifying the liquid sample in the array, detecting a signal emitted by at least one of the probes, and identifying the genetic expression profile in response to the signal.