PERICARP DNA EXTRACTION AND MATRILINEAGE DETERMINATION

This disclosure concerns the separation of pericarp tissue from surrounding tissues in grain. Some embodiments concern the isolation of high-purity pericarp DNA from a grain plant that reflects the genotype of the maternal parent of the grain plant, such that the isolated DNA may be used in a PCR-ba...

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Bibliographische Detailangaben
Hauptverfasser: PIERCE AMY, HAMILTON JENNIFER, HEATH NITIGNA, PATTERSON THOMAS G, RAPIER BRANDON, CANU ANA PAULA, RAIMONDI JUAN PABLO
Format: Patent
Sprache:eng
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Zusammenfassung:This disclosure concerns the separation of pericarp tissue from surrounding tissues in grain. Some embodiments concern the isolation of high-purity pericarp DNA from a grain plant that reflects the genotype of the maternal parent of the grain plant, such that the isolated DNA may be used in a PCR-based genotyping assay.