METHOD FOR IMPROVED TRANSFORMATION USING AGROBACTERIUM

Methods to increase transformation frequency in plants when using Agrobacterium as the transformant are described. The methods include exposing plant cells to Agrobacterium cells in a liquid medium containing a surfactant. Some methods include exposing the plant cells to continuous light after expos...

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1. Verfasser:	MILLER PAUL DAVID
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods to increase transformation frequency in plants when using Agrobacterium as the transformant are described. The methods include exposing plant cells to Agrobacterium cells in a liquid medium containing a surfactant. Some methods include exposing the plant cells to continuous light after exposure to the Agrobacterium cells. Examples of plants useful with these methods include maize plants (e.g., immature maize embryos).