Thermal Cycler For PCR

An assembly for cycling vials of reaction mixtures through a series of temperature excursions is provided. The assembly can include a sample block for receiving the vials, a plurality of thermoelectric devices, a heat sink, and a clamping mechanism positioned to clamp the thermoelectric devices betw...

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Bibliographische Detailangaben
Hauptverfasser: FERRARA KEITH S, WITTMER CHARLES M, NOREIKS RICHARD W, FAWCETT ADRIAN, OLSEN DOUGLAS E, WIDOMSKI JOHN R, HETHERINGTON PAUL M, ATWOOD JOHN G
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:An assembly for cycling vials of reaction mixtures through a series of temperature excursions is provided. The assembly can include a sample block for receiving the vials, a plurality of thermoelectric devices, a heat sink, and a clamping mechanism positioned to clamp the thermoelectric devices between the sample block and heatsink. The assembly can also include a thermal connector having a first end and a second end, the first end in close contact with the sample block and the second end in close contact with the heatsink to provide a thermal path between the sample block and the heatsink, the thermal connector being positioned to reduce thermal gradients across the sample block. The assembly can further include means for connecting the assembly to a computing apparatus for controlling the temperature excursions of the assembly.