Process for PEGylation of Proteins

Process for PEGylation of Proteins

The present invention relates to a process for improving pegylation reaction yield of r-metHuG-CSF comprising conjugating r-metHuG-CSF to a PEG aldehyde at a free amine moiety at the N terminal end on the G-CSF in presence of a reducing agent in a pegylation buffer solution comprising a polyol havin...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: MOHE NIKHIL UMESH, PALIWAL DINESH KUMAR, PATIL NILESH DAGDU, SAKSENA DIVYA LAL, ZAWAR SAGAR SATYANARAYAN, PAWAR DIGAMBER SHRIPATI, KHARE ARUNA, RANA DILIP KUMAR, CHANDRAKESAN MURALIDHARAN, SHEKHAWAT RAKESH, MALPURE PANKAJ PRABHAKAR, TARUR RADHAKRISHNAN VENKATSUBRAMANIAN
Format: Patent
Sprache:eng
Schlagworte:
CHEMISTRY
METALLURGY
ORGANIC CHEMISTRY
PEPTIDES
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Beschreibung
Zusammenfassung:The present invention relates to a process for improving pegylation reaction yield of r-metHuG-CSF comprising conjugating r-metHuG-CSF to a PEG aldehyde at a free amine moiety at the N terminal end on the G-CSF in presence of a reducing agent in a pegylation buffer solution comprising a polyol having the formula CnH2n+2On where n is from 3 to 6, or a carbohydrate, or a derivative thereof wherein the concentration of said polyol or carbohydrate or derivative thereof is in the range of 0.1% to 10% w/w.