Method, Program, and System for Normalizing Gene Expression Amounts
The present invention aims at presenting novel means for analyzing and correcting gene expression amounts. There is provided a gene expression amount normalizing method in which the number of cells in a sample is obtained by measuring a repeated sequence present in a substantially fixed proportion i...
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Zusammenfassung: | The present invention aims at presenting novel means for analyzing and correcting gene expression amounts. There is provided a gene expression amount normalizing method in which the number of cells in a sample is obtained by measuring a repeated sequence present in a substantially fixed proportion in a genome contained in the sample, and the number of cells obtained is used as an index for normalizing gene expression amounts obtained from the same sample. For example, a DNA sample 33 and an RNA sample 34 are obtained from the same sample 32, the DNA sample 33 is used as a sample for obtaining the number of cells, and the RNA sample 34 is used as a sample for obtaining the gene expression amounts, whereby the number of cells contained in the sample 32 and the gene expression amounts relating to the same sample 32 can be obtained. Therefore, by converting the gene expression amounts to values per a fixed number of cells, the gene expression amounts can be normalized to values which can be compared with those obtained by other gene expression analyses. |
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