Automated transient image cytometry

A method, system, and instrument for automatically measuring transient activity in cells uses image time sequences to identify transients in cells. Preferably, the transient activity is stimulated or provoked in synchronism with acquisition of the image time sequences. A cell mask is applied to each...

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Bibliographische Detailangaben
Hauptverfasser: INGERMANSON RANDALL S, CHARLOT DAVID J, PRICE JEFFREY H, MCDONOUGH PATRICK M
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:A method, system, and instrument for automatically measuring transient activity in cells uses image time sequences to identify transients in cells. Preferably, the transient activity is stimulated or provoked in synchronism with acquisition of the image time sequences. A cell mask is applied to each image of an image time sequence in order to localize the transient activity with respect to each cell. Localization enables cell-by-cell analysis of properties of the transient activity.