PRODUCTION OF HETEROLOGOUS PROTEINS OR PEPTIDES

A method of producing a flagellin-based chimeric protein includes culturing a B. halodurans BhFD05 ( hag, fliD, wprA, alp, apr, vpr, asp) strain deposited under Accession Number 41533 at the NCIMB. The strain is caused to express and secrete high levels of a flagellin-based chimeric protein into an...

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Hauptverfasser:	BERGER ELDIE, GERBER ISAK BARTHOLOMEUS, DU PLESSIS ERIKA MARGARETE, CRAMPTON MICHAEL CRAIG, LOUW MAUREEN ELIZABETH
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Zusammenfassung:	A method of producing a flagellin-based chimeric protein includes culturing a B. halodurans BhFD05 ( hag, fliD, wprA, alp, apr, vpr, asp) strain deposited under Accession Number 41533 at the NCIMB. The strain is caused to express and secrete high levels of a flagellin-based chimeric protein into an extracellular growth medium. The flagellin-based chimeric protein comprises a heterologous peptide (i) inserted in-frame into a flagellin variable region which is flanked on its N-terminal side by an N-terminal fragment of a flagellin polypeptide and, optionally, flanked on its C-terminal side by a C-terminal fragment of a flagellin polypeptide, or (ii) fused to the C-terminal of a flagellin polypeptide.