T-STRUCTURE INVASIVE CLEAVAGE ASSAYS, CONSISTENT NUCLEIC ACID DISPENSING, AND LOW LEVEL TARGET NUCLEIC ACID DETECTION

The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also rela...

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Bibliographische Detailangaben
Hauptverfasser: WU KWOK, FLEMING CHRIS, CHEHAK LUANNE, ISZCZYSZYN WALTER, ALLAWI HATIM TAYSIR, LAW SCOTT M, LYAMICHEV VICTOR A, ELAGIN VECHESLAV A
Format: Patent
Sprache:eng
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Zusammenfassung:The present invention relates to systems, methods and kits for low-level detection of nucleic acids, detecting at least two different viral sequences in a single reaction vessel, and increasing the dynamic range of detection of a viral target nucleic acid in a sample. The present invention also relates to T-structure invasive cleavage assays, as well as T-structure related target dependent non-target amplification methods and compositions. The present invention further relates to methods, compositions, devices and systems for consistent nucleic acid dispensing onto surfaces.