High Throughput Screening Assay for the TRPM5 Ion Channel

There exists a need in the art for high throughput screening assays that can identify compounds that specifically modulate the activity of fast-acting ion channels, such as TRPM5. Current methods suffer from a lack of sensitivity, low throughput, and are labor intensive. The claimed methods provide...

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Bibliographische Detailangaben
Hauptverfasser:	LEE S. PAUL, YANG QIFENG, BRYANT ROBERT W, BUBER M.N. TULU, PALMER R. KYLE
Format:	Patent
Sprache:	eng
Schlagworte:	CHEMISTRY COMBINATORIAL CHEMISTRY DERIVATIVES THEREOF INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING METALLURGY NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PHYSICS SUGARS TESTING
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Beschreibung
Zusammenfassung:	There exists a need in the art for high throughput screening assays that can identify compounds that specifically modulate the activity of fast-acting ion channels, such as TRPM5. Current methods suffer from a lack of sensitivity, low throughput, and are labor intensive. The claimed methods provide fluorescent assays with an optical readout that gives rapid readout of the results, has a high signal to noise background ratio, are easy to use, can be modified for automation and miniaturization, and provide verification that a compound specifically modulates TRPM5.