METHOD AND KIT FOR UNIVERSAL VERIFICATION OF ENZYME ACTIVITY AND PROTEIN DIGESTION

A method of assessing enzyme function or protein stability is disclosed. A sample having a protein therein is contacted with an enzyme that is suspected of being capable of catalyzing digestion of the protein in order to form a mixture. First and second aliquots are removed from the mixture at first...

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Bibliographische Detailangaben
Hauptverfasser:	SOMIARI RICHARD IDEM, SOMIARI STELLA BASSEY, RUSSELL STEPHEN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method of assessing enzyme function or protein stability is disclosed. A sample having a protein therein is contacted with an enzyme that is suspected of being capable of catalyzing digestion of the protein in order to form a mixture. First and second aliquots are removed from the mixture at first and second times, respectively, and protein digestion is evaluated using a detection reagent to calculate a change in absorbance between first and second aliquots. The change in absorbance provides an index of enzyme activity, enzyme capability, and protein stability.