RECOVERY AND PURIFICATION OF B-PHYCOERYTHRIN PRODUCED BY PORPHYRIDIUM CRUENTUM USING TWO-AQUEOUS-PHASE SYSTEMS AND ISOELECTRIC PRECIPITATION

RECOVERY AND PURIFICATION OF B-PHYCOERYTHRIN PRODUCED BY PORPHYRIDIUM CRUENTUM USING TWO-AQUEOUS-PHASE SYSTEMS AND ISOELECTRIC PRECIPITATION

This invention focuses on a novel process in which Porphyridium cruentum biomass first undergoes a stage of cellular disruption and subsequently stages of recovery and purification in order to achieve the purified B-phycoerythin (BFE) protein dye, using isoelectric precipitation and two-aqueous-phas...

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Bibliographische Detailangaben
Hauptverfasser: RITO PALOMARES MARCO ANTONIO, HERNANDEZ MIRELES TANHIA DENYS, BENAVIDES LOZANO JORGE ALEJANDRO
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
ENZYMOLOGY
FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:This invention focuses on a novel process in which Porphyridium cruentum biomass first undergoes a stage of cellular disruption and subsequently stages of recovery and purification in order to achieve the purified B-phycoerythin (BFE) protein dye, using isoelectric precipitation and two-aqueous-phase systems. The steps of recovery and purification include isoelectric precipitation followed by a step of liquid/liquid extraction by means of two-aqueous-phase systems that use polyethylene glycol (PEG) and phosphate salts. The BFE protein dye obtained in the two-aqueous-phase extraction step undergoes an ultrafiltration step in order to remove the polymer (PEG) and to obtain a dye with a purity greater than 4.0 defined as the relationship between the absorbencies at 545 and 280 nm (BFE purity=Abs 545 nm/Abs 280 nm).