PROCESSING SOFT TISSUE, METHODS AND COMPOSITIONS RELATED THERETO

In certain embodiments, the present invention relates to a process for preparing skin removed from a human donor, including a living human donor, and removing cellular components and forming a decellular matrix having as major components collagens and elastins while disinfecting the tissue. In other...

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Bibliographische Detailangaben
Hauptverfasser: NGO MANH-DAN, CARTMELL JEFFREY S, VERSEN RUDIGER VON, SYRING CARINA, KAWAS MICHAEL, SUNWOO MOON HAE, DEPAULA CARL ALEXANDER, LEE ALISON, GOCKE DAVID J, GERTZMAN ARTHUR, TRUNCALE KATHERINE G, SEEMAN RICHARD
Format: Patent
Sprache:eng
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Zusammenfassung:In certain embodiments, the present invention relates to a process for preparing skin removed from a human donor, including a living human donor, and removing cellular components and forming a decellular matrix having as major components collagens and elastins while disinfecting the tissue. In other embodiments, the present invention relates to a process for treating a decellularized soft tissue by freezing the same at a plurality of decreasing temperatures at atmosphere or higher such that there is formation of ice crystals having a size greater than 2.0 and lyophilizing the soft tissue under vacuum to remove the water to less than 6% forming a porous matrix.