Detection System

A method for detecting a target nucleic acid sequence in a sample, by subjecting it to an amplification and taking continuous electrochemical measurements on it during the reaction. The method can be used to determine whet amplification reaction has taken place, to quantitate the amount of target i...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	LESLIE DARIO LYALL, LEE MARTIN ALAN
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A method for detecting a target nucleic acid sequence in a sample, by subjecting it to an amplification and taking continuous electrochemical measurements on it during the reaction. The method can be used to determine whet amplification reaction has taken place, to quantitate the amount of target in the sample or to determine sequence characteristics. disclosed is apparatus for use in the method, comprising (i) an amplification reaction vessel which comprises an electrochemic (ii) means for taking continuous electrochemical measurements on a sample contained in the vessel and (iii) tempertature coat measurement means, wherein the electrochemical cell comprises an element formed from an electrically conducting plastics such as a polymer loaded with an electrically conducting material. Further disclosed is a reaction vessel for use in the appar probe for use in the method and a kit for effecting the method.