METHODS OF CONTROLLING THE SENSITIVITY AND DYNAMIC RANGE OF A HOMOGENEOUS ASSAY

METHODS OF CONTROLLING THE SENSITIVITY AND DYNAMIC RANGE OF A HOMOGENEOUS ASSAY

A method is disclosed for accurately determining the concentration of a target analyte utilizes reagent pairs having different affinity for the target. The different affinity provides distinct binding profiles that can be analyzed to absolutely determine the analyte concentration. The method provide...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: DILLMORE W. SHANNON, WEIDEMAIER KRISTIN
Format: Patent
Sprache:eng
Schlagworte:
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
MEASURING
PHYSICS
TESTING
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Beschreibung
Zusammenfassung:A method is disclosed for accurately determining the concentration of a target analyte utilizes reagent pairs having different affinity for the target. The different affinity provides distinct binding profiles that can be analyzed to absolutely determine the analyte concentration. The method provides an assay system having expanded dynamic range to cover a wider range of analyte concentration and can overcome the hook-effect that commonly exists in homogenous assay systems. The method utilizes distinguishable signals that allows for the analysis of multiple binding profiles and multiplex analysis.