STRAIN OF GENETICALLY REENGINEERED ESCHERICHIA COLI FOR BIOSYNTHESIS OF HIGH YIELD CAROTENOIDS AFTER MUTATION SCREENING

The present invention relates to a strain of Escherichia coli (E. coli M2H) for biosynthesis of high yield carotenoids, the steps for obtaining E. coli M2H comprises: purifying the host strain JCL1613 for pCW9/P2IDI; create plasmids for producing carotenoid genes; transform the plasmids into JCL1613...

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Bibliographische Detailangaben
Hauptverfasser:	LIN CHI-NAN, CHIOU MING-HSI, YANG MING-HAW, LU YIN, WU MEIIAO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a strain of Escherichia coli (E. coli M2H) for biosynthesis of high yield carotenoids, the steps for obtaining E. coli M2H comprises: purifying the host strain JCL1613 for pCW9/P2IDI; create plasmids for producing carotenoid genes; transform the plasmids into JCL1613; utilizing physical method (UV) to induce and improve JCL1613; select the portions with 99% death rate; utilizing 50 mL shake flask incubation; and go through UV screening to obtain the mutated strain of E. coli M2H.