PLUGGED FLOW 2D CHROMATOGRAPHY

The invention provided is a split-free nano-liter flow liquid chromatography system comprising at least a first and a second chromatographic column connected in series, wherein an autosampler is provided and programmed to inject samples and eluent solutions of increasing eluent concentration for gra...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: THEODORSEN SOREN, VORM OLE, SORENSEN OLE TANG, ANDERSEN MICHAEL BARRETT
Format: Patent
Sprache:eng
Schlagworte:
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Beschreibung
Zusammenfassung:The invention provided is a split-free nano-liter flow liquid chromatography system comprising at least a first and a second chromatographic column connected in series, wherein an autosampler is provided and programmed to inject samples and eluent solutions of increasing eluent concentration for gradually eluting analytes from the first column into the second column (without adversely affecting separation in the second column), said solutions segmented from the mobile phase of the liquid chromatography system by air plugs.