OPTIMISATION OF CULTURE CONDITIONS FOR PRODUCTION AND PROCESS OF PURIFICATION OF HIGH MOLECULAR WEIGHT HYALURONIC ACID

The present invention provides an efficient and improved process for the production and purification of high molecular weight hyaluronic acid (HA). One embodiment relates to providing growth conditions for production of high molecular weight HA in Streptococcus zooepidemicus. For example, growth in...

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Bibliographische Detailangaben
Hauptverfasser:	RANGASWAMY VIDHYA, VYAS SANTOSH, JAIN DHARMENDRA, KRISHNA SONTI VENKATA RAMA, KAPAT ARNAB, NATARAJ VEDAPURI, VELANKAR HARSHAD
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides an efficient and improved process for the production and purification of high molecular weight hyaluronic acid (HA). One embodiment relates to providing growth conditions for production of high molecular weight HA in Streptococcus zooepidemicus. For example, growth in medium comprising a lower protein concentration, e.g., 1% casein hydrolyzate, and a higher sugar concentration, e.g., 5% sucrose, gave 5-6 g/L of high molecular weight HA in the range of 3.5-4.0x106 Da. The present invention also provides an efficient process for the purification of HA comprising treatment with silica gel then active carbon, and subsequent diafiltration with less volume of solvent.