Method for detecting reaction of protein and sample

There is provided a method for detecting a reaction of a fluorescently labeled protein and a sample simply, in a short time and at a higher precision. By using an expression system such as an extracellular gene expression system and an extracellular transcription or translation system, a reaction of...

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Bibliographische Detailangaben
1. Verfasser:	OKAMOTO NAOAKI
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	There is provided a method for detecting a reaction of a fluorescently labeled protein and a sample simply, in a short time and at a higher precision. By using an expression system such as an extracellular gene expression system and an extracellular transcription or translation system, a reaction of expressing a protein from a constructed vector is performed. At this time, a fluorescently labeled amino acid is introduced into the expression system in the form of a tRNA having the fluorescently labeled amino acid. With expression of a protein, the fluorescently labeled amino acid is incorporated into the protein, and a fluorescently labeled protein (hereafter, referred to as fluorescently labeled protein) is produced. The fluorescently labeled protein and a sample S are mixed to prepare a mixed solution, and FCS measurement is performed. Based on a measured value of the FCS, the presence or absence of a reaction is detected.