Affinity marker for purification of proteins

The present invention relates to an affinity marker comprising a FLAG-domain, which contains at least one FLAG-tag, and a Streptavidin-binding domain (Strep-domain), which contains at least two Strep-tags, a protein containing this affinity marker, a nucleic acid which codes for it, a vector or a ce...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	UEFFING MARIUS, GLOECKNER CHRISTIAN J
Format:	Patent
Sprache:	eng
Schlagworte:	CHEMISTRY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING METALLURGY ORGANIC CHEMISTRY PEPTIDES PHYSICS TESTING
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Beschreibung
Zusammenfassung:	The present invention relates to an affinity marker comprising a FLAG-domain, which contains at least one FLAG-tag, and a Streptavidin-binding domain (Strep-domain), which contains at least two Strep-tags, a protein containing this affinity marker, a nucleic acid which codes for it, a vector or a cell containing the affinity marker, method for the purification of a protein produced in a cell using this affinity marker, and the use of the affinity marker for the purification of a protein produced in a cell.