METHODS OF CREATING CONSUMABLE STRAINS AND COMPOSITIONS THEREOF

Consumable biotech strain improvement products are presented, as well as methods of preparation and using them. The technology is based on reversible, single-crossover insertion vectors, such as plasmids or phage. Because the single crossover event is reversible in the absence of drug selection, the...

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Bibliographische Detailangaben
Hauptverfasser: WEBER J. M, REEVES ANDREW, BRIKUN IGOR A
Format: Patent
Sprache:eng
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Zusammenfassung:Consumable biotech strain improvement products are presented, as well as methods of preparation and using them. The technology is based on reversible, single-crossover insertion vectors, such as plasmids or phage. Because the single crossover event is reversible in the absence of drug selection, the products cannot be maintained in a useful form without knowledge of the drug selection agent. Consumable strain improvement products can be constructed with 1st generation reverse engineering protections, having at least 25%-75% of the effectiveness of the equivalent traditional (permanent) strain improvement product under laboratory condition.