Chimeric Human Growth Hormone Derived From The Placenta And Pituitary Isoform And Processes For Obtaining Said Chimera

Our invention relates to a new way of preparing coding DNA constructs, by making a chimera of various coding region isoforms. For example, our method has been successfully used to make a new hGH-NV cDNA chimera having SEQ ID NO: 3, by combining the cDNA isoforms of human placenta and pituitary RNA e...

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Hauptverfasser:	MAITI DIPANWITA, KHARE GANESH ARUNA, NIPIIADKAR MILIND PRABHAKAR, BORBIIUIYA AHMED MONSUR, MISIIRA SHRIKANT, RAO LAXMI SRINIVAS
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	Our invention relates to a new way of preparing coding DNA constructs, by making a chimera of various coding region isoforms. For example, our method has been successfully used to make a new hGH-NV cDNA chimera having SEQ ID NO: 3, by combining the cDNA isoforms of human placenta and pituitary RNA encoding human growth hormone. Our invention also relates to a process for increasing the production of recombinant human growth hormone by inserting a plurality of promoter-coding region-termination sequences into a vector in tandem. For example, our method has been successfully used to approximately double the yield of recombinant growth hormone in transformed cells.