Methods and compositions for the detection of Chlamydia trachomatis

The present invention provides novel methods for determining the presence or absence of Chlamydia in a patient, as well as diagnostic kits useful in practicing the methods of the invention. The methods of the invention are based on nucleic acid amplification reactions to detect both Chlamydia genomi...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	GREWING THOMAS, EICKHOFF MEIKE
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides novel methods for determining the presence or absence of Chlamydia in a patient, as well as diagnostic kits useful in practicing the methods of the invention. The methods of the invention are based on nucleic acid amplification reactions to detect both Chlamydia genomic and cryptic plasmid sequences. In one embodiment, the methods involve using nucleic acid primers to specifically amplify the Chlamydia trachomatis ompA gene and cryptic plasmid. These methods provide both enhanced reliability and sensitivity of detection, thereby providing an accurate determination of the presence or absence of Chlamydia trachomatis in a patient.