Method for preparating t-20 peptide by high cell density cultivation of recombinant e. coli containing t-20 peptide coding gene

The present invention relates to a method for preparing a T-20 peptide by the high-cell-density cultivation of recombinant E. coli containing a T-20 peptide-coding gene, as well as a method for isolating and purifying the T-20 peptide with high purity from E. coli where the T-20 peptide have been ex...

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Bibliographische Detailangaben
Hauptverfasser:	JANG HONG K, CHOI NAM H, YOO JAE W, TAK KEON T, KANG CHOONG K
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA DERIVATIVES THEREOF ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PEPTIDES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention relates to a method for preparing a T-20 peptide by the high-cell-density cultivation of recombinant E. coli containing a T-20 peptide-coding gene, as well as a method for isolating and purifying the T-20 peptide with high purity from E. coli where the T-20 peptide have been expressed. According to the present invention, the recombinant E. coli containing the T-20 peptide-coding gene can be cultured at a high cell concentration, and the T-20 peptide can be isolated and purified with high purity from said recombinant E. coli, thereby making it possible to obtain the inventive T-20 peptide with high industrial value economically.