Nucleic acid amplification primers for pcr-based clonality studies

The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearr...

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Bibliographische Detailangaben
Hauptverfasser:	EVANS PAUL ANTHONY S, MACINTYRE ELIZABETH A, PARREIRA ANTONIO, KNEBA MICHAEL, SAN MIQUEL JESUS F, GARZIA SANZ RAMON, SCHUURING EDUARDUS MARIA D, HUMMEL MICHAEL, BASTARD CHRISTIAN, LAVENDER FRANCES L, VAN DONGEN JACOBUS JOHANNES M, SMITH JOHN L, MORGAN GARETH J
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES DERIVATIVES THEREOF ENZYMOLOGY MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING NUCLEIC ACIDS NUCLEOSIDES NUCLEOTIDES ORGANIC CHEMISTRY PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS SUGARS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to PCR-based clonality studies for among others early diagnosis of lymphoproliferative disorders. Provided is a set of nucleic acid amplification primers comprising a forward primer, or a variant thereof, and a reverse primer, or a variant thereof, capable of amplifying a rearrangement selected from the group consisting of a VH-JH IGH rearrangement, a DH-JH IGH rearrangement, a VK-JK IGK rearrangement, a VK/intron-Kde IGK rearrangement, a Vlambda-Jlambda IGL rearrangement, a Vbeta-Jbeta TCRB rearrangement, a Dbeta-Jbeta TCRB rearrangement, a Vgamma-Jgamma TCRG rearrangement, a Vdelta-Jdelta TCRD rearrangement, a Ddelta-Ddelta TCRD rearrangement, a Ddelta-Jdelta TCRD rearrangement, a Vdelta-Ddelta TCRD rearrangement, or a translocation selected from t(11; 14)(BCL1-IGH) and t(14;18)(BCL2-IGH). The primers can be used in PCR-based clonality studies for early diagnosis of lymphoproliferative disorders and detection of minimal residual disease (MRD). Also provided is a kit comprising at least one set of primers of the invention.