Process for producing glucose dehydrogenase

A glucose dehydrogenase complex is produced by culturing an Escherichia bacterium, which is introduced with DNAs encoding the alpha-subunit and the beta-subunit of glucose dehydrogenase of Burkhorderia cepacia in an expressible form, and in which expression of the ccm system (cytochrome c maturation...

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Bibliographische Detailangaben
Hauptverfasser:	KUROSAKA KEISUKE, HOSHIJIMA MITSUHIRO, YAMAOKA HIDEAKI, KAWASE SHIDO
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A glucose dehydrogenase complex is produced by culturing an Escherichia bacterium, which is introduced with DNAs encoding the alpha-subunit and the beta-subunit of glucose dehydrogenase of Burkhorderia cepacia in an expressible form, and in which expression of the ccm system (cytochrome c maturation system) is enhanced, to express the aforementioned DNAs and produce a glucose dehydrogenase complex, and collecting the complex.