Vectors for plant transformation and methods of use

The present invention is directed to a vector for identifying read-through of non-T-DNA in a T-DNA vector. In one embodiment, the vector provides a visually detectable change in the normal appearance of transformants wherein read-through has occurred. In another embodiment, the vector also provides...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser:	BERTAIN SEAN M, WILKINSON JACK Q, MCBRIDE KEVIN E, LASSNER MICHAEL
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FISHING FORESTRY HUMAN NECESSITIES HUNTING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW PLANTS OR PROCESSES FOR OBTAINING THEM PLANT REPRODUCTION BY TISSUE CULTURE TECHNIQUES PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention is directed to a vector for identifying read-through of non-T-DNA in a T-DNA vector. In one embodiment, the vector provides a visually detectable change in the normal appearance of transformants wherein read-through has occurred. In another embodiment, the vector also provides for expression of a readily detectable fluorescent protein that allows for the early detection and elimination of transformants wherein read-through has occurred. In a further aspect, the present invention is directed to a method for detecting read-through of non-T-DNA in plants transformed with a T-DNA vector. In another aspect, the present invention is directed to a method for producing a transgenic plant containing a polynucleotide of interest but being substantially free of non-T-DNA.