Integration vectors

The invention relates to integration vectors for modifying a target genomic region comprising, in a 5' to 3' direction, a splice acceptor site, a 3' hybrid recognition site, and a marker sequence (i.e., a 5' gene trap vector); or alternatively comprising, in a 5' to 3'...

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Bibliographische Detailangaben
1. Verfasser:	FINNEY ROBERT E
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to integration vectors for modifying a target genomic region comprising, in a 5' to 3' direction, a splice acceptor site, a 3' hybrid recognition site, and a marker sequence (i.e., a 5' gene trap vector); or alternatively comprising, in a 5' to 3' direction, a marker sequence; a 5' hybrid recognition site; and a splice donor site (i.e., a 3' gene trap vector). The integration vector, upon insertion into the target genomic region is capable of producing a recombinant RNA transcript that is comprised of a hybrid recognition site for a selection molecule. The hybrid recognition site of recombinant RNA produced from insertion of the 5' gene trap vector is comprised of a 5' hybrid recognition site derived from genomic sequence and a 3' hybrid recognition site derived from vector sequence. The hybrid recognition site of recombinant RNA produced from insertion of the 3' gene trap vector is comprised of a 5' hybrid recognition site derived from vector sequence and a 3' hybrid recognition site derived from genomic sequence. The selection molecule selects recombinant cells comprising the integration vector inserted within the target genomic region.