Identification and purification of higher order transcription complexes from transgenic non-human animals

The invention relates to transgenes comprising DNA encoding for epitope-tagged TATA-box binding protein (TBP), the production of transgenic animals which express epitope-tagged TBP and use thereof for affinity purification of transcription factors and transcription complexes from a variety of eukary...

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Bibliographische Detailangaben
Hauptverfasser:	MEISTERERNST MICHAEL, POLITES GREG, KIRSCHBAUM BERND, BERGLUND ERICK
Format:	Patent
Sprache:	eng
Schlagworte:	AGRICULTURE ANIMAL HUSBANDRY BEER BIOCHEMISTRY CARE OF BIRDS, FISHES, INSECTS CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE FISHING FORESTRY HUMAN NECESSITIES HUNTING INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING NEW BREEDS OF ANIMALS ORGANIC CHEMISTRY PEPTIDES PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROCESSES USING MICROORGANISMS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS REARING OR BREEDING ANIMALS, NOT OTHERWISE PROVIDED FOR SPIRITS TESTING TRAPPING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to transgenes comprising DNA encoding for epitope-tagged TATA-box binding protein (TBP), the production of transgenic animals which express epitope-tagged TBP and use thereof for affinity purification of transcription factors and transcription complexes from a variety of eukaryotic tissues and cell-types.