Method for cultivation of the nitrile-hydratase-producing strain Rhodococcus rhodochrous M33

The invention relates to a method for cultivation of the nitrile-hydratase-producing strain Rhodococcus rhodochrous M33, using a culture medium which is based on a 12 to 60 mM phosphate buffer, covers the demand of the cells for phosphorus and maintains the pH in the range of 5.5 to 9.0 during culti...

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Bibliographische Detailangaben
Hauptverfasser:	KOSOLIN S.W, YANENKO A.S, SINGIREZEV I.N, WORONIN S.P, LORENZ JOSEF, DEBABOV V.G
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method for cultivation of the nitrile-hydratase-producing strain Rhodococcus rhodochrous M33, using a culture medium which is based on a 12 to 60 mM phosphate buffer, covers the demand of the cells for phosphorus and maintains the pH in the range of 5.5 to 9.0 during cultivation, and to which acetic acid is added in doses as the new source of carbon after consumption of the initially supplied quantity of glucose. The invention also relates to the culture medium used in the method.