Process for production of large amount of penicillin V acylase

Process for production of large amount of penicillin V acylase

The present invention relates to a recombinant plasmid of FIG. 1 , wherein (1) is pET-26b(+) cloning/expression region with SEQ ID No. 1 cloned between BamH I site 198 and Nde I site 288, (2) is lac I coding sequence, (3) is pBR322 origin, (4) is Kan coding sequence, and (5) is f1 origin; a recombin...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: SIVARAMAN HEPHZIBAH, PUNDLE ARCHANA V, BRANNIGAN JAMES A, SURESH CHERAVAKKATTU G, DODSON GEORGE G
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The present invention relates to a recombinant plasmid of FIG. 1 , wherein (1) is pET-26b(+) cloning/expression region with SEQ ID No. 1 cloned between BamH I site 198 and Nde I site 288, (2) is lac I coding sequence, (3) is pBR322 origin, (4) is Kan coding sequence, and (5) is f1 origin; a recombinant E. Coli strain PTA 2456; and lastly, a process for the production of large amount of Penicillin V acylase using recombinant E. Coli strain PTA 2456.