Protein bioarray on silane-modified substrate surface

The invention provides a method of producing a protein bioarray that includes providing a substrate comprising a solid support and a surface modification layer bound to the solid support. The surface modification layer includes a first moiety having the structure -Si-R1 and a second moiety having the structure -Si-L-R2, wherein R1 is a chemically inert moiety selected from the group consisting of C3 to C30 alkyl and benzyl optionally substituted with 1 to 5 halogen atoms, L is a linking group, and R2 is a chemically inert hydrophilic moiety. The method of producing the protein bioarray further includes providing at least two solutions, wherein each solution contains a probe protein. In the method, each of the solutions is then deposited at its own discrete site on the substrate. The probe proteins deposited on the substrate become non-covalently bound to the substrate.