Hybridization normalization methods

Hybridization normalization methods

The present invention includes methods of normalizing hybridization reactions that are designed to select normalization control genes, specifically 5'-3'-, and middle portions of the these genes, that hybridize similarly to a probe array and that produce the most consistently linear curve...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: BEAZER-BARCLAY YASMIN, ANTONELLIS KRISTEN J, ELASHOFF MICHAEL, WHITLEY MARYANN, BROWN EUGENE L, SCHERF UWE, JELINSKY SCOTT A
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS OR TEST PAPERS THEREFOR
CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES
ENZYMOLOGY
MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS
METALLURGY
MICROBIOLOGY
MUTATION OR GENETIC ENGINEERING
PROCESSES OF PREPARING SUCH COMPOSITIONS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:The present invention includes methods of normalizing hybridization reactions that are designed to select normalization control genes, specifically 5'-3'-, and middle portions of the these genes, that hybridize similarly to a probe array and that produce the most consistently linear curve of hybridization signal over a range of normalization control gene segment concentrations. These methods have applicability across a broad spectrum of hybridization formats.