Method of preparing cross-linked enzyme aggregates

The invention relates to a method of preparing cross-linked enzyme aggregates (CLEAs) comprising the steps of a) precipitating a dissolved enzyme present in a liquid medium: b) cross-linking with a cross-linker, and c) optionally, washing the cross-linked enzyme aggregate. According to the invention...

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Bibliographische Detailangaben
Hauptverfasser:	CAO LINQIU, VAN RANTWIJK FREDERIK, LOPEZ SERRANO PALOMA, SHELDON ROGER ARTHUR
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	The invention relates to a method of preparing cross-linked enzyme aggregates (CLEAs) comprising the steps of a) precipitating a dissolved enzyme present in a liquid medium: b) cross-linking with a cross-linker, and c) optionally, washing the cross-linked enzyme aggregate. According to the invention at least one treatment is performed chosen from the group consisting of I) performing step a) in the presence of a compound selected from the group consisting of i) a crown ether, and ii) a surfactant, and II) performing step c) with a second liquid differing in organic composition from the liquid.