In vitro translation system

In vitro translation (IVT) systems and methods for increased expression of proteins from linear templates, using GamS, are provided. The proteins may be full length or protein fragments. The IVT system may be used in batch or continuous mode. The GamS may be used as GamS nucleic acid template, crude...

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Bibliographische Detailangaben
Hauptverfasser: CURTIS DAMIAN E, BOWMAN KRISTA K, CANCILLA MICHAEL ROBERT, CIANCIO MARGIE, ZHAN HANGJUN, BUCKLEY DOUGLAS IWEN, LEE JAE MOON
Format: Patent
Sprache:eng
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Zusammenfassung:In vitro translation (IVT) systems and methods for increased expression of proteins from linear templates, using GamS, are provided. The proteins may be full length or protein fragments. The IVT system may be used in batch or continuous mode. The GamS may be used as GamS nucleic acid template, crude protein fraction, or purified protein product. The IVT system using GamS component may be employed in a high-throughput mode. The ability to predict expressible protein or fragments, and activity and solubility of a large-scale protein expression product based on the results obtained from high-throughput, small-scale IVT expression product is also provided.