Circuit for imposing voltages on the electrodes of trays used in the chef pulsed fied elctrophoresis system

Circuit to clamp the voltages in the electrodes of the CHEF system chambers of Pulsed Field Gel Electrophoresis (PFGE) which is formed by two identical clamping circuits that are connected to a power supply through an alternator and only one of the two circuits receives electric power at the same ti...

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Bibliographische Detailangaben
Hauptverfasser: ROJAS ANA MARIA RIVERON, HERRERA ISIDRON JOSE ALFREDO, BLANCO MARIA DOLORES NOA, RAMOS CARLOS ALBERTO CANINO, CANOVAS LILIA LOPEZ
Format: Patent
Sprache:eng
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Zusammenfassung:Circuit to clamp the voltages in the electrodes of the CHEF system chambers of Pulsed Field Gel Electrophoresis (PFGE) which is formed by two identical clamping circuits that are connected to a power supply through an alternator and only one of the two circuits receives electric power at the same time. Each clamping circuit is formed by several resistors and diodes connected in series to form a voltage divider. Voltage repeaters are connected to the nodes formed in the union of two resistors. Each repeater is connected to a pair of electrodes that should be polarized at the same potential. Diodes are introduced to correct small errors in the voltage pattern applied to the electrodes. The circuit is able to maintain the potential of each electrode in front of the variations of conductivity that occur inside the chamber during the electrophoresis. This way each imposition circuit generates a homogeneous electric field of same value and different direction in a PFGE chamber of the CHEF system. Chambers with different number, disposition and separation among the electrodes can be polarized with this circuit.