Plastid transformation

The present invention relates to the field of plastid transformation. The invention provides transformation vectors and methods to obtain transplastomic plants or algae having a transformed plastid comprising the steps of introducing into plastids a recombinant nucleic acid molecule or vector, and t...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: HEIFETZ PETER, GU WEINING, DEFRAMOND ANIC, BOUDREAU ERIC
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The present invention relates to the field of plastid transformation. The invention provides transformation vectors and methods to obtain transplastomic plants or algae having a transformed plastid comprising the steps of introducing into plastids a recombinant nucleic acid molecule or vector, and two phases of selection with first selection phase using a non-lethal compound and a second selection phase using a lethal compound . Alternatively, the dual selection method is conducted simultaneously using a lower concentration of the lethal compound.