Methods for in situ generation of nucleic acid arrays

Methods of producing nucleic acid arrays using an in situ nucleic acid synthesis protocol are provided, where the in situ nucleic acid synthesis protocol includes a plurality of cycles, each of which includes: (I) a monomer attachment step; and (II) a functional group, e.g., 5'OH, generation st...

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Hauptverfasser:	WOODWORTH ROBERT E, LEPROUST ERIC M
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES ENZYMOLOGY LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MUTATION OR GENETIC ENGINEERING PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PROCESSES OF PREPARING SUCH COMPOSITIONS SPIRITS THEIR RELEVANT APPARATUS TRANSPORTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	Methods of producing nucleic acid arrays using an in situ nucleic acid synthesis protocol are provided, where the in situ nucleic acid synthesis protocol includes a plurality of cycles, each of which includes: (I) a monomer attachment step; and (II) a functional group, e.g., 5'OH, generation step, the latter of which includes: (a) oxidation and (b) deblocking substeps. A feature of the subject methods is that the deblocking substep is performed by contacting a substrate surface with a deblocking solution made up of a deblocking agent present in an organic solvent, wherein the organic solvent is toluene or another organic solvent having substantially the same vapor pressure. Also provided are the arrays produced using the subject methods, as well as methods for use of the arrays and kits that include the same.