Nutritional mixture and method for early identification and count of gram-negative organisms

The present invention is related with Microbiology and particularly with a nutrient mixture and a procedure for the identification and the early count of Gram-negative organisms. This mixture allows the development of five different colony colors in the organisms to be detected, and the appearance o...

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Hauptverfasser: TSORAEVA ANNA, QUESADA MUNIZ VIVIAN DE JESUS
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:The present invention is related with Microbiology and particularly with a nutrient mixture and a procedure for the identification and the early count of Gram-negative organisms. This mixture allows the development of five different colony colors in the organisms to be detected, and the appearance of three colored fluorescent emissions, the appearance of three different colors halos and zones with opaque precipitates surrounding such colonies. Those factors, together with the color changes in the medium, allow a differentiation with a high sensibility and specificity level. The mixture include specific relations of the mixtures of tryptophan rich protein fractions, free tryptophan, organic or inorganic salts, color or fluorescence providing substances, cellulose and hemi cellulose and other components which provide from 1 to 2 layers in the composition.