Expression system for recombinant proteins

A continuous fermentation process has been developed in Pichia pastoris (P. pastoris) in order to produce large quantities of recombinant human proteins. High expression levels have been demonstrated in continuous production of the enzyme by P. pastoris with a constitutive promoter in a 1.5-liter wo...

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Bibliographische Detailangaben
Hauptverfasser:	SCHILLING BERNHARD M, GOODRICK JASON C, WAN NICK, HOPPE HENRY
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY FERMENTATION OR ENZYME-USING PROCESSES TO SYNTHESISE A DESIREDCHEMICAL COMPOUND OR COMPOSITION OR TO SEPARATE OPTICAL ISOMERSFROM A RACEMIC MIXTURE METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS VINEGAR WINE
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Beschreibung
Zusammenfassung:	A continuous fermentation process has been developed in Pichia pastoris (P. pastoris) in order to produce large quantities of recombinant human proteins. High expression levels have been demonstrated in continuous production of the enzyme by P. pastoris with a constitutive promoter in a 1.5-liter working volume fermenter using either glucose or glycerol as the carbon source. The fermentation could be extended for long periods of time with a excellent steady-state protein concentration and cell densities achieved. No proteolytic degradation of the enzyme was seen in the continuous fermentation mode.