Feedback-regulated expression system and uses thereof

Feedback-regulated expression system and uses thereof

A feedback-regulated expression system comprising a nucleic acid construct comprising a first polynucleotide encoding an elicitin operably linked to a first plant promoter comprising at least one E. coli lac operator (LacO) located between the promoter TATA box and the translation initiation site of...

Ausführliche Beschreibung

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Bibliographische Detailangaben
Hauptverfasser: DATTAROY TOMAL, HUNT ARTHUR G, LI QINGSHUN
Format: Patent
Sprache:eng
Schlagworte:
BEER
BIOCHEMISTRY
CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
VINEGAR
WINE
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Beschreibung
Zusammenfassung:A feedback-regulated expression system comprising a nucleic acid construct comprising a first polynucleotide encoding an elicitin operably linked to a first plant promoter comprising at least one E. coli lac operator (LacO) located between the promoter TATA box and the translation initiation site of the first polynucleotide, wherein the first plant promoter is constitutive; and a second polynucleotide encoding an E. coli lac repressor (LacI) operably linked to a PR gene is described. the feedback-regulated expression system is used to generate transgenic plants that have enhanced resistance to plant pathogens.