METHOD AND DEVICE FOR FIXING MICRO-AND/OR NANO-OBJECTS

The invention relates to a method for obtaining recombinant HbsAg. According to said method, recombinant methylotrophic yeast cells that are capable of expressing HbsAg are broken down using a high-pressure homogenizer and the HBsAg is extracted from the resulting cell debris. The inventive method i...

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Bibliographische Detailangaben
Hauptverfasser:	JENTSCH WINFRIED, SCHMUCKER ULRICH, ZUBTSOV MIKHAIL
Format:	Patent
Sprache:	eng
Schlagworte:	CHEMICAL OR PHYSICAL LABORATORY APPARATUS FOR GENERAL USE CHEMICAL OR PHYSICAL PROCESSES, e.g. CATALYSIS OR COLLOIDCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING METALLURGY PERFORMING OPERATIONS PHYSICAL OR CHEMICAL PROCESSES OR APPARATUS IN GENERAL PHYSICS TESTING THEIR RELEVANT APPARATUS TRANSPORTING
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Beschreibung
Zusammenfassung:	The invention relates to a method for obtaining recombinant HbsAg. According to said method, recombinant methylotrophic yeast cells that are capable of expressing HbsAg are broken down using a high-pressure homogenizer and the HBsAg is extracted from the resulting cell debris. The inventive method is characterised by a high product yield per g cell dry weight and is therefore a considerable improvement on known methods for obtaining HBsAg from micro-organisms.