Protein expression and structure solution using specific fusion vectors

A recombinant protein comprised of an amino acid sequence of a motor protein, a target protein of interest, and optionally, a linker sequence between the two proteins, is disclosed. Also disclosed are a DNA sequence encoding such a recombinant protein, a vector expressing such a recombinant protein,...

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Hauptverfasser: NIEMANN HARTMUT H, KNETSCH MENNO L. W, MANSTEIN DIETMAR J, KULL JON F
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creator NIEMANN HARTMUT H
KNETSCH MENNO L. W
MANSTEIN DIETMAR J
KULL JON F
description A recombinant protein comprised of an amino acid sequence of a motor protein, a target protein of interest, and optionally, a linker sequence between the two proteins, is disclosed. Also disclosed are a DNA sequence encoding such a recombinant protein, a vector expressing such a recombinant protein, a host cell transformed with such a vector, a method for producing such a recombinant protein, and methods for purification, crystallization and structure elucidation of such a recombinant protein. In preferred embodiments of the invention, the motor protein is a member of the kinesin or myosin superfamilies, or an analog, fragment or derivative thereof.
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subjects BEER
BIOCHEMISTRY
CHEMISTRY
COMBINATORIAL CHEMISTRY
COMPOSITIONS THEREOF
CULTURE MEDIA
ENZYMOLOGY
INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES
LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES
MEASURING
METALLURGY
MICROBIOLOGY
MICROORGANISMS OR ENZYMES
MUTATION OR GENETIC ENGINEERING
ORGANIC CHEMISTRY
PEPTIDES
PHYSICS
PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS
SPIRITS
TESTING
VINEGAR
WINE
title Protein expression and structure solution using specific fusion vectors
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