Protein expression and structure solution using specific fusion vectors

A recombinant protein comprised of an amino acid sequence of a motor protein, a target protein of interest, and optionally, a linker sequence between the two proteins, is disclosed. Also disclosed are a DNA sequence encoding such a recombinant protein, a vector expressing such a recombinant protein,...

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Bibliographische Detailangaben
Hauptverfasser:	NIEMANN HARTMUT H, KNETSCH MENNO L. W, MANSTEIN DIETMAR J, KULL JON F
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMBINATORIAL CHEMISTRY COMPOSITIONS THEREOF CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES LIBRARIES, e.g. CHEMICAL LIBRARIES, IN SILICOLIBRARIES MEASURING METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING ORGANIC CHEMISTRY PEPTIDES PHYSICS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	A recombinant protein comprised of an amino acid sequence of a motor protein, a target protein of interest, and optionally, a linker sequence between the two proteins, is disclosed. Also disclosed are a DNA sequence encoding such a recombinant protein, a vector expressing such a recombinant protein, a host cell transformed with such a vector, a method for producing such a recombinant protein, and methods for purification, crystallization and structure elucidation of such a recombinant protein. In preferred embodiments of the invention, the motor protein is a member of the kinesin or myosin superfamilies, or an analog, fragment or derivative thereof.