Establishment of cell lines with persistent expression of a green fluorescent protein (GFP) using a pIRES/EGFP DNA vector construct

The present invention provides a vector stably transforming cells. The vector comprises the green florescent protein (GFP) from Aequorea Victoria as a marker. The invention also provides stably transfected cell lines which may be used to assess real-time biolgical processes, including tumor cell mig...

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Bibliographische Detailangaben
Hauptverfasser:	FILLMORE HELLEN, SHURM JOHN S, GILLIES GEORGE T, BROADDUS WILLIAM C
Format:	Patent
Sprache:	eng
Schlagworte:	BEER BIOCHEMISTRY CHEMISTRY COMPOSITIONS OR TEST PAPERS THEREFOR COMPOSITIONS THEREOF CONDITION-RESPONSIVE CONTROL IN MICROBIOLOGICAL ORENZYMOLOGICAL PROCESSES CULTURE MEDIA ENZYMOLOGY INVESTIGATING OR ANALYSING MATERIALS BY DETERMINING THEIRCHEMICAL OR PHYSICAL PROPERTIES MEASURING MEASURING OR TESTING PROCESSES INVOLVING ENZYMES, NUCLEICACIDS OR MICROORGANISMS METALLURGY MICROBIOLOGY MICROORGANISMS OR ENZYMES MUTATION OR GENETIC ENGINEERING PHYSICS PROCESSES OF PREPARING SUCH COMPOSITIONS PROPAGATING, PRESERVING OR MAINTAINING MICROORGANISMS SPIRITS TESTING VINEGAR WINE
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Beschreibung
Zusammenfassung:	The present invention provides a vector stably transforming cells. The vector comprises the green florescent protein (GFP) from Aequorea Victoria as a marker. The invention also provides stably transfected cell lines which may be used to assess real-time biolgical processes, including tumor cell migration.