Nucleic acid assays employing universal arrays

Hybridization assays, as well as kits, primers and arrays for use in practicing the same, are provided. In the subject assays, a population of tagged target nucleic acids generated from a population of tagged gene specific primers is contacted with an array of tag complements under hybridization con...

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Hauptverfasser: SIMONENKO PETER N, CHENCHIK ALEX, TCHAGA GRIGORIY S
Format: Patent
Sprache:eng
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Beschreibung
Zusammenfassung:Hybridization assays, as well as kits, primers and arrays for use in practicing the same, are provided. In the subject assays, a population of tagged target nucleic acids generated from a population of tagged gene specific primers is contacted with an array of tag complements under hybridization conditions and the presence of any resultant hybridized tag target nucleic acid-tag complement structures is detected. The subject arrays find use in a number of different applications, e.g. differential gene expression analysis.